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portada Chimeric Vaccine Design Against Melioidosis via Subtractive Proteomics de Salman Akhtar Mohammad Kalim Ahmad Khan(Ks Omniscriptum Publishing)
Type
Physical Book
Publisher
Author
Language
English
Format
Paperback
ISBN13
9786208417253

Chimeric Vaccine Design Against Melioidosis via Subtractive Proteomics de Salman Akhtar Mohammad Kalim Ahmad Khan(Ks Omniscriptum Publishing)

Ahmad Khan Mohammad Kalim,Akhtar Salman,Maurya Shalini (Author) · Ks Omniscriptum Publishing · Paperback

Chimeric Vaccine Design Against Melioidosis via Subtractive Proteomics de Salman Akhtar Mohammad Kalim Ahmad Khan(Ks Omniscriptum Publishing) - Ahmad Khan Mohammad Kalim,Akhtar Salman,Maurya Shalini

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Synopsis "Chimeric Vaccine Design Against Melioidosis via Subtractive Proteomics de Salman Akhtar Mohammad Kalim Ahmad Khan(Ks Omniscriptum Publishing)"

Subtractive proteomics and reverse vaccinology were employed to design a chimeric multi-epitope vaccine against melioidosis, a severe infectious disease caused by the multidrug-resistant bacterium, Burkholderia pseudomallei. From 21 non-redundant pathogen proteomes, four proteins were selected as vaccine candidates based on essentiality, virulence, non-homology to humans, structural availability, subcellular localization, and antigenicity. The major histocompatibility complex class I, II, and B-cell epitopes were predicted and evaluated for immunogenicity, solubility, allergenicity, and hydrophilicity. A chimeric vaccine was constructed using the epitopes, adjuvants, linkers, and PADRE sequences. Docking and molecular dynamics simulations confirmed interactions between HLA alleles and TLR4, indicating the potential to elicit an immune response, demonstrating the utility of computational tools in developing vaccines against B. pseudomallei.

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