Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.
From the reviews of the second edition:
"The 2nd edition … resulted in an extraordinarly helpful tool for lab practice and for developing multiples approaches in order to successfully perform these type of analysis. … authors made each chapter a valuable tool for beginners and senior investigators who want to explore DNA methylation analysis in their biological systems. … used for advanced researchers in order to complement their work with other methodologies. … This is definitively a volume that must be present at each lab working in this cutting-edge field … ." (Marcia Manterola, European Journal of Histochemistry, October, 2009)
