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portada Dna Modification Detection Methods (Springer Protocols Handbooks)
Type
Physical Book
Publisher
Author
Language
English
Pages
200
Format
Paperback
ISBN13
9781071612316
Edition No.
1

Dna Modification Detection Methods (Springer Protocols Handbooks)

Yuan Bi-Feng (Author) · Humana · Paperback

Dna Modification Detection Methods (Springer Protocols Handbooks) - Yuan Bi-Feng

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Synopsis "Dna Modification Detection Methods (Springer Protocols Handbooks)"

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.

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