Essential Genes and Genomes: Methods and Protocols
Zhang, Ren
Synopsis "Essential Genes and Genomes: Methods and Protocols"
1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9 Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat 2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor 3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes Bert Kwanten, Jasper Neggers, and Dirk Daelemans 4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans Lauren Wensing and Rebecca S. Shapiro 5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens Luke DeHart, Oliver P. Yockey, and Jesse Bakke 6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines Kohei Nishimura and Tatsuo Fukagawa 7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen Xihao Liao, Xin-Hui Xing, and Chong Zhang 8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang 9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum Miguel Carda-Diéguez and Carmen Amaro 10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung 11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing Alex J. McCarthy and Peter W. Taylor 12. Using Genome Scale Mutant Libraries to Identify Essential Genes Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue 13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing Alejandro R. Walker and Robert C. Shields 14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems Gayatri Sharma and Patrick D. Curtis 15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus
