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portada Lentivirus Gene Engineering Protocols
Type
Physical Book
Publisher
Author
Language
English
Pages
228
Format
Paperback
Dimensions
25.4x17.8x1.3 cm
Weight
0.43 kg.
ISBN13
9781493956531
Edition No.
0002
Categories

Lentivirus Gene Engineering Protocols

Federico, Maurizio (Author) · Humana · Paperback

Lentivirus Gene Engineering Protocols - Federico, Maurizio

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Synopsis "Lentivirus Gene Engineering Protocols"

Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.

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