Name: membrane protein protocols: expression, purification, and characterization
Price: 147.97 EUR
Availability: InStock
Author: Selinsky, Barry S.
ISBN: 9781617373763

portada membrane protein protocols: expression, purification, and characterization

membrane protein protocols: expression, purification, and characterization

Type

Physical Book

Illustrated by

Barry S. Selinsky

Publisher

Humana

Author

Selinsky, Barry S.

Year

2010

Language

English

Pages

334

Format

Paperback

Dimensions

22.9 x 15.2 x 2.0 cm

Weight

0.51 kg.

ISBN

1617373761

ISBN13

9781617373763

Synopsis "membrane protein protocols: expression, purification, and characterization"

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Customers reviews

Have you read this book? Login to add your review.

More customer reviews

0% (0)
0% (0)
0% (0)
0% (0)
0% (0)

Questions and Answers about the Book

Do you have a question about the book? Login to be able to add your own question.

Opinions about Bookdelivery

More customer reviews

membrane protein protocols: expression, purification, and characterization - Selinsky, Barry S.

Synopsis "membrane protein protocols: expression, purification, and characterization"

Customers reviews

Frequently Asked Questions about the Book

Is the book original?

In what language is the book written?

What is the format of this book?

Questions and Answers about the Book

Opinions about Bookdelivery