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portada Microbial Transposon Mutagenesis: Protocols and Applications
Type
Physical Book
Publisher
Author
Language
English
Pages
183
Format
Paperback
ISBN13
9781493995721
Edition No.
1
Categories

Microbial Transposon Mutagenesis: Protocols and Applications

Ricke, Steven C. ; Park, Si Hong ; Davis, Morgan L. (Author) · Humana · Paperback

Microbial Transposon Mutagenesis: Protocols and Applications - Ricke, Steven C. ; Park, Si Hong ; Davis, Morgan L.

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Synopsis "Microbial Transposon Mutagenesis: Protocols and Applications"

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

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