Name: pcr cloning protocols
SKU: 3406452
Price: 146.49 EUR
Availability: InStock
ISBN: 9780896039698

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Type

Physical Book

Publisher

Springer

Language

English

Pages

460

ISBN

0896039692

ISBN13

9780896039698

Synopsis "pcr cloning protocols"

pcr cloning protocols, second edition, updates and expands bruce whites best-selling pcr cloning protocols (1997) with the newest procedures for dna cloning and mutagenesis. here the researcher will find readily reproducible methods for all the major aspects of pcr use, including pcr optimization, computer programs for pcr primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance pcr and gc-rich template amplification. also included are both conventional and novel enzyme-free and restriction site-free procedures to clone pcr products into a range of vectors, as well as state-of-the-art protocols to facilitate dna mutagenesis and recombination, and to clone the challenging uncharacterized dna flanking a known dna fragment.

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