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portada Cell-Free Circulating Dna: Purification and Analysis Techniques
Type
Physical Book
Illustrated by
Publisher
Author
Language
English
Pages
296
Format
Hardcover
Dimensions
22.9x15.2x1.8 cm
Weight
0.57 kg.
ISBN13
9789811244674
Categories

Cell-Free Circulating Dna: Purification and Analysis Techniques

Kristina Warton (Illustrated by) · Goli Samimi (Illustrated by) · World Scientific Publishing Company · Hardcover

Cell-Free Circulating Dna: Purification and Analysis Techniques - Warton, Kristina ; Samimi, Goli

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Synopsis "Cell-Free Circulating Dna: Purification and Analysis Techniques "

Circulating cell-free DNA is poised to transform cancer diagnosis and care; however, it carries technical challenges such as low abundance, fragmentation and sensitivity to biospecimen handling. Development of clinically useful assays hinges on understanding the unique technical aspects of working with cell-free DNA as a substrate. This book provides an in-depth summary of the technical issues impacting cell-free DNA purification from blood plasma and analysis in a cancer context, including design of PCR assays, sequencing library preparation and methylation analysis. Emerging fields such as extracellular vesicles and blood nucleases are also covered, as well as basic biology and considerations pertinent to biobanking.

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