1. Cell Migration in Three Dimensions Coert Margadant Part I: The Cell Biology of Cell Migration 2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood 3. Probing the ER-Focal Adhesion Link during Cell Migration Noemi Antonella Guadagno and Cinzia Progida 4. Mapping the Localization of Proteins within Filopodia Using FiloMap Guillaume Jacquemet 5. Extended Methods for 2D Confinement Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel 6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63 Bong Hwan Sung and Alissa M. Weaver 7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis 8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila Maik C. Bischoff and Sven Bogdan 9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells Antonius L. van Boxtel 10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp 11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila Sofia J. Araújo and Marta Llimargas 12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland Edouard Hannezo and Colinda L.G.J. Scheele 13. Analysis of Integrin-Dependent Melanoblast Migration during Development Amanda Haage and Guy Tanentzapf Part III: Cell Migration in Cancers and the Tumor Microenvironment 14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro 15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion Jian Zhang and Cynthia A. Reinhart-King 16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids &n
