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portada e. coli plasmid vectors: methods and applications
Type
Physical Book
Illustrated by
Publisher
Author
Year
2010
Language
English
Pages
316
Format
Paperback
Dimensions
22.9x15.2x1.9 cm
Weight
0.49 kg.
ISBN
1617373915
ISBN13
9781617373916
Categories

e. coli plasmid vectors: methods and applications

Nicola Casali (Illustrated by) · Andrew Preston (Illustrated by) · Humana · Paperback

e. coli plasmid vectors: methods and applications - Casali, Nicola ; Preston, Andrew

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Synopsis "e. coli plasmid vectors: methods and applications"

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

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