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portada Genetic Engineering: Principles and Methods
Type
Physical Book
Publisher
Author
Language
English
Pages
232
Format
Paperback
Dimensions
23.4x15.6x1.3 cm
Weight
0.36 kg.
ISBN13
9781475787986
Categories

Genetic Engineering: Principles and Methods

Setlow, Jane K. (Author) · Springer · Paperback

Genetic Engineering: Principles and Methods - Setlow, Jane K.

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Synopsis "Genetic Engineering: Principles and Methods"

Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).

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