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portada Methods for Fragments Screening Using Surface Plasmon Resonance
Type
Physical Book
Publisher
Author
Language
English
Pages
129
Format
Hardcover
ISBN13
9789811615351
Edition No.
1

Methods for Fragments Screening Using Surface Plasmon Resonance

Zaheer Sameer Mahmood (Author) · Springer · Hardcover

Methods for Fragments Screening Using Surface Plasmon Resonance - Zaheer Sameer Mahmood

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Synopsis "Methods for Fragments Screening Using Surface Plasmon Resonance"

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.

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